{"id":1202,"date":"2020-03-29T12:08:13","date_gmt":"2020-03-29T06:38:13","guid":{"rendered":"http:\/\/www.fineflowtech.com\/?p=1202"},"modified":"2020-12-21T09:49:27","modified_gmt":"2020-12-21T04:19:27","slug":"laboratory-minifor-reactor","status":"publish","type":"post","link":"https:\/\/www.fineflowtech.com\/?p=1202","title":{"rendered":"MINIFOR Bioreactor"},"content":{"rendered":"
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Lab-Scale Production of Rhamnolipid Biosurfactants with MINIFOR Benchtop Bioreactor<\/strong><\/h3>\n<\/div>\n
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LAMBDA MINIFOR Benchtop Bioreactor used for the Lab-Scale Production of Biosurfactant Rhamnolipids secreted by Pseudomonas aeruginosa\u00a0A3<\/p>\n<\/div>\n

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Biosurfactants (microbial-produced surfactants)<\/strong>\u00a0are a promising alternative for chemically-synthesized surfactants in industrial applications. They are more effective, selective, environmentally friendly, and stable than many synthetic surfactants. To produce a large quantity of biosurfactant-<\/span>rhamnolipid (RL),\u00a0<\/strong>researchers from the Baghdad University used wild strains of<\/span>\u00a0<\/strong>Pseudomonas aeruginosa isolated from the local region of Iran. Isolated strains were cultivated in the Batch mode using\u00a0<\/em>LAMBDA MINIFOR 7L fermentor for the production of RL.\u00a0<\/strong><\/p>\n

\"Lab-Scale<\/p>\n

Reference:\u00a0Faqri, A.M.A ; Hayder, N.H & Hashim, A. J. Lab-scale production of Rhamnolipid by Pseudomonas aeruginosa A3 and study its synergistic effect with certain antibiotics against some pathogenic bacteria. Iraqi Journal of Agricultural Sciences \u20132019:50(5):1290-1301.<\/a><\/em><\/p>\n

After 120 h, the surface tension of the solution reached 27.2 mN\/m, with\u00a06<\/strong>7% of emulsification activity and biomass 2.7 g\/l.<\/strong>\u00a0The\u00a0RL concentration was 16 g\/l<\/strong>. Using Silica-gel chromatography, Thin Layer Chromatography (TLC) and Fourier Transform Infrared Spectroscopy (FTIR) for spectral analysis, the biosurfactant mixture obtained was characterized mainly of both mono- and di-rhamnolipids. Cytotoxicity assays of mono-RL were done revealing a\u00a0synergistic effect with antibiotics<\/strong>\u00a0such as: ampicillin against\u00a0Staphylococcus aureus\u00a0<\/em>and\u00a0Serratia marcescens<\/em>, with cefotaxime against\u00a0Echerichia coli<\/em>\u00a0and\u00a0Klebsiella pneumoniae<\/em>, same as with tetracycline.<\/p>\n

LAMBDA\u00a0<\/strong>MINIFOR\u00a0<\/strong>Bioreactor<\/strong>\u00a0was easy to handle and all important cultural conditions could be measured and controlled.<\/h3>\n

Ergonomic and easy handling. Why?<\/strong><\/h4>\n